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Example Plates


Biotechnology and Bioinformatics utilize microtitre plates as a standard format for reference and unknown sample storage and processing, instrumentation, analysis, aggregation and retrieval of data. Since microtitre plates play such a central role, BioXing has developed a highly customizable interactive and enhanced microtitre plate component, called BxMicroTitrePlate, which can be easily incorporated into .NET Windows applications. The component is useful for rendering an interactive microtitre plate graphic in applications that perform operations or measurements using microtitre plates. In particular for instrument control, for data acquisition monitoring and analysis summary, multiple plate comparisons, as a navigation tool, and as an interface to facilitate accessing and integrating related well content information. 

Standard 96-well, 384-well and 1536-well plate sizes are selectable and other sizes are easily defined. Important features of this component are multiple shapes to represent different types of well content such as samples, standards and controls and gray-scale and gradient interior well fill patterns to represent the magnitude of known and measured values respectively. Wells may also contain, annotation text, graphs of 2D data and images. The intent is to provide a view that can not only be used interactively, but can also be used to convey multiple pieces of information.

Due to its flexible design the control can be used to represent generic interactive arrays of 1 x m (single row), n x1 (single column) or n x m where n and m are the number of rows and columns in the array and through the use of the customization properties the array does not have to have the appearance of microtitre plate. This is useful, for example, when representing rectangular fraction collectors or representing collections of data (numbers, graphs or images) for comparison.

Example views with detailed descriptions are contained in the downloadable Views document and in the Example Plates section.

This workflow figure shows an example of how the BxMicroTitrePlate control is integrated into analysis protocols. The plate is used at the beginning of the workflow to represent the structure/content to be analyzed and then is used to show analyzed results. Subsequently, additional data/information can be interactively retrieved or saved from/to persistent storage. The Magnifier view, a selectable plate control option, is used to compare the selected (yellow gradient) well with its replicate wells (blue gradient). The gray-scale coloring of the gradients shows the magnitude of the intensity measurement along with the numeric annotation within the well. This is useful for large plates when the annotation is too small to read and when the plate wells contain graphs and images (refer to Example Plates section). The plate can be copied to the clipboard and incorporated into printed results and views.





The following figure shows an example application containing the BxMicroTitrePlate control with the options selected for showing embedded and popup magnifier viewers. Two-dimensional data linked to the selected well is rendered as an XY Graph using BxPlot2D in the viewers.  A database and a collection of data files are connected to the plate using an internal .NET DataTable object that contains data and/or links associated with each well. The plate image can be copied to the clipboard and printed within reports containing results and views. In addition the graph with its annotation in the embedded viewer can also be copied and printed.  Multiple wells can be selected for displaying in the viewers.



















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Last modified: May 2017